MALDI Mass Spectrometry on High-Density Droplet Arrays: Matrix Deposition, Selective Removal, and Recrystallization.

ABSTRACT: High-density droplet arrays are emerging as a powerful tool for high-throughput bioanalytical applications. These arrays are formed of thousands of nanoliter droplets, which can be analyzed by various optical and spectroscopic methods as well as label-free matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). However, special precautions are required for the massive number of small droplets, particularly in the deposition of matrix compounds. Here, we introduce a new workflow for the analytical preparation of an array comprising 6048 droplets, which significantly improves the intensity of the MALDI-MS signals. We deposited matrix compounds in a custom-made sublimation chamber followed by a recrystallization step to achieve significant signal intensity increases for thre

SUBMITTER: Berlanda SF 

PROVIDER: S-EPMC11487676 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature