Cross-Species Applications of Peptide Substrate Reporters to Quantitative Measurements of Kinase Activity.

ABSTRACT: Peptide substrate reporters are short chains of amino acids designed to act as substrates for enzymes of interest. Combined with capillary electrophoresis and laser-induced fluorescence detection (CE-LIF), they are powerful molecular tools for quantitative measurements of enzyme activity even at the level of single cells. Although most peptide substrate reporters have been optimized for human or murine cells in health-related applications, their performance in nonmammalian organisms remains largely unexplored. In this study, we evaluated three peptide substrate reporters for protein kinase B (PKB) in two eukaryotic microbes, Dictyostelium discoideum and Tetrahymena thermophila, which are evolutionarily distant from mammals and from each other yet express PKB homologues. All t

SUBMITTER: Fan MJ 

PROVIDER: S-EPMC11487760 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature