Reduced circulating CD63⁺ extracellular vesicle levels associate with atherosclerosis in hypercholesterolaemic mice and humans.

ABSTRACT:

Aims

The association and co-isolation of low-density lipoproteins (LDL) and extracellular vesicles (EVs) have been shown in blood plasma. Here we explore this relationship to better understand the role of EVs in atherogenesis.

Methods and results

Wild type (WT), PCSK9^-/-, and LDLR^-/- C57BL/6 mice were used in this study. Eleven week-old male mice were fed high-fat diet (HFD) for 12 weeks or kept on normal diet until old age (22-months). Cardiac function was assessed by ultrasound, cholesterol was quantified with a colorimetric kit and circulating EVs were measured using flow cytometry. Plaques were analysed post-mortem using Oil-Red-O staining of the aortic arch. EVs were measured from platelet free blood plasma samples of normal and hypercholesterolaem

SUBMITTER: Kestecher BM 

PROVIDER: S-EPMC11487797 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature