Highly integrated, self-powered and activatable bipedal DNA nanowalker for imaging of base excision repair in living cells.

ABSTRACT: DNA walkers have attracted considerable attention in biosensing and bioimaging. Compared with the conventional single leg-based DNA walker, the bipedal DNA walker has remarkable advantages, with improved sensitivity and fast kinetics, and can work efficiently in a crowded cellular environment. However, most reported bipedal DNA walkers are powered by exogenous supplementation, and elaborate DNA sequence designs, auxiliary additives or extra carriers are often needed. A highly integrated bipedal DNA walker that can address robustness, sensitivity and consistency issues in a single system is highly desirable but remains a great challenge. We herein report a novel bipedal DNA nanowalker system through simple assembly of a DNA substrate, hairpin functionalized-AuNPs (AuNPs-H2), and a blocked M

SUBMITTER: Lai R 

PROVIDER: S-EPMC11487878 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature