H Antigen expression modulates epidermal Keratinocyte Integrity and differentiation.

ABSTRACT:

Background

ABO blood group antigens (ABH antigens) are carbohydrate chains glycosylated on epithelial and red blood cells. Recent findings suggest reduced ABH expression in psoriasis and atopic dermatitis, a chronic inflammatory skin disease with retained scale. H antigen, a precursor for A and B antigens, is synthesized by fucosyltransferase 1 (FUT1). Desmosomes, critical for skin integrity, are known to require N-glycosylation for stability. We investigate the impact of H antigens, a specific type of glycosylation, on desmosomes in keratinocytes.

Method

Primary human keratinocytes were transfected with FUT1 siRNA or recombinant adenovirus for FUT1 overexpression. Cell adhesion and desmosome characteristics and their underlying mechanisms were analyzed.

Result

The kn

SUBMITTER: Jin SP 

PROVIDER: S-EPMC11487879 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature