Toll-like receptors ligand immunomodulators for the treatment congenital diaphragmatic hernia.

ABSTRACT:

Background

Congenital diaphragmatic hernia (CDH) is a rare disease that affects the development of the diaphragm, leading to abnormal lung development. Unfortunately, there is no established therapy for CDH. Retinoic acid pathways are implicated in the ethology of CDH and macrophages are known to play a role in repairing organ damage.

Methods

We have analyzed the effect of several Toll like receptor (TLR) ligands in the nitrofen-induced CDH model in pregnant rats widely used to study this disease and in the G2-GATA4^Cre;Wt1^fl/fl CDH genetic mice model. Morphometric and histological studies were carried out. Immune cell infiltration was assayed by immunochemistry and immunofluorescence and retinoic pathway gene expression analyzed in vivo and in vitro in

SUBMITTER: Vallejo-Cremades M 

PROVIDER: S-EPMC11487987 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature