Spatial transcriptomic sequencing reveals immune microenvironment features of <i>Mycobacterium tuberculosis</i> granulomas in lung and omentum.

ABSTRACT: Granulomas are a key pathological feature of tuberculosis (TB), characterized by cell heterogeneity, spatial composition, and cellular interactions, which play crucial roles in granuloma progression and host prognosis. This study aims to analyze the transcriptome profiles of cell populations based on their spatial location and to understand the core transcriptome characteristics of granuloma formation and development. Methods In this study, we collected four clinical biopsy samples including Mycobacterium tuberculosis (Mtb) infected lung (MTB-L) and omentum tissues (MTB-O), as well as two lung and omentum biopsies from non-TB patients. The tissues were analyzed by spatial transcriptomics to create a spatial atlas. Utilizing cell enrichment scores and intercellular communicati

SUBMITTER: Qiu X 

PROVIDER: S-EPMC11488093 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature