PET imaging of microglia in Alzheimer's disease using copper-64 labeled TREM2 antibodies.

ABSTRACT: Triggering receptor expressed on myeloid cells 2 (TREM2) plays an essential role in microglia activation and is being investigated as a potential therapeutic target for modulation of microglia in several neurological diseases. In this study, we present the development and preclinical evaluation of ⁶⁴Cu-labeled antibody-based PET radiotracers as tools for non-invasive assessment of TREM2 expression. Furthermore, we tested the potential of an antibody transport vehicle (ATV) that binds human transferrin receptor to facilitate transcytosis of TREM2 antibody-based radiotracers to the CNS and improve target engagement. Methods: A TREM2 antibody with an engineered transport vehicle (ATV:4D9) and without (4D9) were covalently modified with pNCS-benzyl-NODAGA and labeled w

SUBMITTER: Shojaei M 

PROVIDER: S-EPMC11488106 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature