A novel method of Francisella infection of epithelial cells using HeLa cells expressing fc gamma receptor.

ABSTRACT:

Background

Francisella tularensis, the causative agent of tularemia, is a facultative intracellular bacterium. Although the life cycle of this bacterium inside phagocytic cells (e.g., macrophages, neutrophils) has been well analyzed, the difficulty of gene silencing and editing genes in phagocytic cells makes it difficult to analyze host factors important for the infection. On the other hand, epithelial cell lines, such as HeLa, have been established as cell lines that are easy to perform gene editing. However, the infection efficiency of Francisella into these epithelial cells is extremely low.

Methods

In order to facilitate the molecular biological analysis of Francisella infection using epithelial cells, we constructed an efficient infection model of F. tularensis subsp.

SUBMITTER: Nakamura T 

PROVIDER: S-EPMC11488177 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature