PYCR1 promotes liver cancer cell growth and metastasis by regulating IRS1 expression through lactylation modification.

ABSTRACT:

Background

Liver cancer (LC) is among the deadliest cancers worldwide, with existing treatments showing limited efficacy. This study aimed to elucidate the role and underlying mechanisms of pyrroline-5-carboxylate reductase 1 (PYCR1) as a potential therapeutic target in LC.

Methods

Immunohistochemistry and Western blot were used to analyse the expression of PYCR1 in LC cells and tissues. EdU assays, colony-forming assays, scratch wound healing assays, Transwell assays, nude mouse xenograft models and nude mouse lung metastasis models were used to detect the growth and metastasis abilities of LC cells. Transcriptome sequencing was used to search for downstream target genes regulated by PYCR1, and metabolomics was used to identify the downstream metabolites regulated by PYCR1.

SUBMITTER: Wang H 

PROVIDER: S-EPMC11488319 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature