Data Analysis of Dynamics in Protein Solutions Using Quasi-Elastic Neutron Scattering─Important Insights from Polarized Neutrons.

ABSTRACT: Protein dynamics play a vital role in biology. Quasi elastic neutron scattering (QENS) is an ideal method to access these dynamics. To isolate protein dynamics, it is important to separate the signal of the buffer and the protein. Normally data analysis is performed based on the assumption that the scattering spectrum is incoherent. To observe the full range of protein dynamics, it is necessary to perform the experiments in solution. This solution is usually a fully deuterated buffer, while the protein remains protonated. It is generally assumed that subtracting the buffer contribution removes all coherent signal from the measured spectrum, and the rest can be considered as purely incoherent. Up until recently, there was no way to experimentally verify this assumption. Polarized QENS exper

SUBMITTER: Sarter M 

PROVIDER: S-EPMC11488478 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature