Spray inoculation and image analysis-based quantification of powdery mildew disease severity on pea leaves.

ABSTRACT: Pea (Pisum sativum) is an important agricultural legume crop, but powdery mildew disease caused by the biotrophic fungus Erysiphe pisi regularly limits its annual yield. Assays to evaluate the efficacy of potential antifungal compounds or resistance genes for disease control require a simple fungal inoculation method that provides control over the initial inoculum concentration and enables uniform inoculum distribution within a leaf and across replicates as well as a method for the quantitative assessment of disease severity. Here, we present an easy spray inoculation method for the uniform distribution of a defined concentration of E. pisi conidia on the leaves of pea plants and a semi-automated image analysis-based quantification of disease symptoms. The uniformity i

SUBMITTER: Ray P 

PROVIDER: S-EPMC11489057 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature