Fingerprinting Tertiary Structure in Complex RNAs Using Single-Molecule Correlated Chemical Probing.

ABSTRACT: Single-molecule correlated chemical probing (smCCP) is an experimentally concise strategy for characterizing higher-order structural interactions in RNA. smCCP data yield rich, but complex, information about base pairing, conformational ensembles, and tertiary interactions. To date, through-space communication specifically measuring RNA tertiary structure has been difficult to isolate from structural communication reflective of other interactions. Here, we introduce mutual information as a filtering metric to isolate tertiary structure communication contained within smCCP data and use this strategy to characterize the structural ensemble of the SAM-III riboswitch. We identified an smCCP fingerprint that is selective for states containing a tertiary structure that forms concurrently with co

SUBMITTER: Tan AC 

PROVIDER: S-EPMC11489888 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature