Progesterone increases metabolism via the pentose phosphate pathway in bovine uterine epithelial cells.

ABSTRACT:

Background

During early pregnancy, glucose is essential for the uterine epithelium and the developing embryo. In cows, progesterone increases the secretion of glucose into the uterine lumen. The uterine epithelium can convert glucose to fructose, but other fates of glucose in the uterine epithelium have been sparsely investigated. Therefore, our objective was to investigate how progesterone influences glucose metabolism in immortalized bovine uterine epithelial (BUTE) cells.

Methods

BUTE cells were grown to 80% confluence and treated with vehicle (DMSO) or 10 µM progesterone for 24 h. Cells were collected and analyzed. Immunohistochemistry was performed on endometrial samples collected from the bovine endometrium on days 1 and 11 of the reproductive cycle.

Results

Pro

SUBMITTER: Berg MD 

PROVIDER: S-EPMC11490429 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature