LAT1 supports mitotic progression through Golgi unlinking in an amino acid transport activity-independent manner.

ABSTRACT: Amino acid transporters play a vital role in cellular homeostasis by maintaining protein synthesis. L-type amino acid transporter 1 (LAT1/SLC7A5/CD98lc) is a major transporter of large neutral amino acids in cancer cells because of its predominant expression. Although amino acid restriction with various amino acid analog treatments is known to induce mitotic defects, the involvement of amino acid transporters in cell division remains unclear. In this study, we identified that LAT1 is responsible for mitotic progression in a transport activity-independent manner. LAT1 knockdown activates the spindle assembly checkpoint, leading to a delay in metaphase. LAT1 maintains proper spindle orientation with confinement of the lateral cortex localization of the NuMA protein, which mediates the pullin

SUBMITTER: Yanagida S 

PROVIDER: S-EPMC11490712 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature