Evolutionary and molecular basis of ADP-ribosylation reversal by zinc-dependent macrodomains.

ABSTRACT: Dynamic ADP-ribosylation signaling is a crucial pathway that controls fundamental cellular processes, in particular, the response to cellular stresses such as DNA damage, reactive oxygen species, and infection. In some pathogenic microbes, the response to oxidative stress is controlled by a SirTM/zinc-containing macrodomain (Zn-Macro) pair responsible for establishment and removal of the modification, respectively. Targeting this defence mechanism against the host's innate immune response may lead to novel approaches to support the fight against emerging antimicrobial resistance. Earlier studies suggested that Zn-Macros play a key role in the activation of this defence. Therefore, we used phylogenetic, biochemical, and structural approaches to elucidate the functional properties of these e

SUBMITTER: Ariza A 

PROVIDER: S-EPMC11490716 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature