DetectCilia: An R Package for Automated Detection and Length Measurement of Primary Cilia.

ABSTRACT:

Background and objective

The primary cilium is a small protrusion found on most mammalian cells. It acts as a cellular antenna, being involved in various cell signaling pathways. The length of the primary cilium affects its function. To study the impact of physical or chemical stimuli on cilia, their lengths must be determined easily and reproducibly.

Methods

We have developed and evaluated an open-source R package called detectCilia to detect and measure primary cilia automatically. As a case study to demonstrate the capability of our tool, we compared the influence of 4 different cell culture media compositions on the lengths of primary cilia in human chondrocytes. These media compositions include (1) insulin-transferrin-selenium (ITS); (2) ITS and dexamethasone (Dexa); (3

SUBMITTER: Budde-Sagert K 

PROVIDER: S-EPMC11490958 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature