Development of multiplex real-time PCR for simultaneous detection of common fungal pathogens in invasive mycoses.

ABSTRACT:

Background

Fungi are common opportunistic pathogens that pose a significant threat to immunocompromised patients, particularly when late detection occurs.

Methods

In this study a multiplex real-time PCR has been developed for simultaneous detection of common fungal pathogens associated with invasive mycoses in a diagnostic setting.

Results

The specificity of the assay was rigorously tested on 40 types of organisms (n = 65), demonstrating 100% specificity. The limit of detection was determined to be 100 pg/μl (10⁶ copies/μl), achievable within a rapid 3-h timeframe. The PCR assay efficiency exhibited a range between 89.77% and 104.30% for each target organism, with linearity falling between 0.9780 and 0.9983.

Conclusion

This multiplex real-time

SUBMITTER: Ismadi YKM 

PROVIDER: S-EPMC11491059 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature