The RNA m⁵C methyltransferase NSUN1 modulates human malaria gene expression during intraerythrocytic development.

ABSTRACT:

Introduction

Plasmodium falciparum is the most damaging malaria pathogen and brings a heavy burden to global health. Host switching and morphological changes in P. falciparum are dependent on an effective gene expression regulatory system. C5 methylation of cytosines is a common RNA modification in eukaryotes, and the NSUN family are essential m⁵C modification executors. Currently, little is known about this family in Plasmodium spp. In this study, we focus on exploring the function of PfNSUN1 protein.

Methods

An efficient CRISPR/Cas9 gene editing technique was applied to construct the PfNSUN1 knockdown strain. The knockdown efficiency was confirmed by growth curves and western blot experiments. The knockdown transcriptome data w

SUBMITTER: Tang R 

PROVIDER: S-EPMC11491294 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature