Facile construction of dual-response super-resolution probes for tracking organelles dynamics.

ABSTRACT: Super-resolution imaging techniques, such as structured illumination microscopy (SIM), have enabled researchers to obtain nanoscale organelle-level outputs in living systems, but they impose additional stringent requirements on fluorescence probes. However, high-performance, custom-designed SIM probes that can explain underlying biological processes remain unavailable. Herein, a customizable engineering toolkit is developed for the facile assembly of SIM probes suitable for subcellular component detection. This toolkit is used to customize a fluorescent molecule, CPC (coumarin-phenylhydrazine-carboxyl), capable of simultaneously monitoring peroxynitrite (ONOO^-) and polarity distribution in mitochondria and lipid droplets (LDs), respectively, through functional ON-OFF mech

SUBMITTER: Liu D 

PROVIDER: S-EPMC11491301 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature