EMF treatment delays mesenchymal stem cells senescence during long-term <i>in vitro</i> expansion by modulating autophagy.

ABSTRACT:

Introduction

Bone marrow mesenchymal stem cells (BMSCs) are widely used in tissue engineering and regenerative medicine as seed cells. Due to low amount in bone marrow, BMSCs must be expanded and cultured in vitro before application. However, the senescence of stem cell caused by long-term in vitro culture greatly limits its efficacy of transplantation.

Methods

In this study, we propose an approach based on electromagnetic fields (EMF) treatment to rejuvenate aged BMSCs due to long-term in vitro culture. Aged BMSCs were treated with sinusoidal EMF (50 Hz, 0.4 mT), and stem cell senescence, cell proliferation, cell differentiation, cell stemness and autophagy level were detected. Additionally, aged BMSCs-laden hydrogels were transplanted into the rat crit

SUBMITTER: Cai W 

PROVIDER: S-EPMC11491334 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature