The impact of cryopreservation on cytokine secretion and polyfunctionality in human PBMCs: a comparative study.

ABSTRACT:

Introduction

Human peripheral blood mononuclear cells (hPBMCs) are widely used in fundamental research and clinical applications as studying their responses to in vitro activation is an effective way to uncover functional alterations and disease associated phenotypes. However, the availability of samples in large numbers at a specific time and location remains challenging, hence they often might preferably be collected and cryopreserved for later analysis. While the effect of cryopreservation on viability and cell surface expression is well established, changes in activity and cytokine secretion still lead to conflicting results as it is often measured in bulk or within the cells.

Methods

Here, we used our platform for dynamic single-cell multiplexed cytokine secretio

SUBMITTER: Linder A 

PROVIDER: S-EPMC11491348 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature