Unraveling the mechanisms of RECQL4-mediated cervical cancer progression through the PI3K/AKT pathway.

ABSTRACT:

Background

RECQL4 is a member of the DNA helicase family and is critical for DNA replication, DNA damage repair, and tumor progression. However, its specific role in cervical cancer remains uncertain.

Methods

In this study, we aimed to investigate the impact of RECQL4 on cervical cancer prognosis using clinical specimens from The Cancer Genome Atlas. We evaluated the malignant effects of RECQL4 through various experimental assays including cell Cell Counting Kit-8, EdU, colony formation, cell cycle analysis, cell apoptosis, scratch, and Transwell assays. We explored the mechanisms of RECQL4-regulated malignancy using analyses of bioinformatics, RNA sequencing data, polymerase chain reaction (PCR), western blotting, and cell immunofluorescence experiments. Furthermore, we val

SUBMITTER: Huo W 

PROVIDER: S-EPMC11491730 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature