Insecticide susceptibility in a planthopper pest increases following inoculation with cultured Arsenophonus.

ABSTRACT: Facultative vertically transmitted symbionts are a common feature of insects that determine many aspects of their hosts' phenotype. Our capacity to understand and exploit these symbioses is commonly compromised by the microbes unculturability and consequent lack of genetic tools, an impediment of particular significance for symbioses of pest and vector species. Previous work had established that insecticide susceptibility of the economically important pest of rice, the brown planthopper Nilaparvata lugens, was higher in field-collected lineages that carry Ca. Arsenophonus nilaparvatae. We established Ca. A. nilaparvatae into cell-free culture and used this to establish the complete closed genome of the symbiont. We transformed the strain to express GFP and reintroduced it to N. lugens to t

SUBMITTER: Cai T 

PROVIDER: S-EPMC11491930 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature