The role of lipid oxidation pathway in reactive oxygen species-mediated cargo release from liposomes.

ABSTRACT: Reactive oxygen species (ROS)-mediated photooxidation is an efficient method for triggering a drug release from liposomes. In addition to the release of small molecules, it also allows the release of large macromolecules, making it a versatile tool for controlled drug delivery. However, the exact release mechanism of large macromolecules from ROS-sensitive liposomes is still unclear. There are no studies on the effect of lipid oxidation on the release of cargo molecules of different sizes. By using HPLC-HRMS method we analyzed the oxidation products of ROS-sensitive DOTAP lipid in phthalocyanine-loaded DOTAP:Cholesterol:DSPE-PEG liposomes after 630 nm light irradiation of different durations. Shorter illumination time (1-2 minutes) led to the formation of hydroperoxides and vic-alcohols pr

SUBMITTER: Lem O 

PROVIDER: S-EPMC11491990 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature