Regulation of cagA-Helicobacter on gastric PIM2 expression in gastric cancer.

ABSTRACT:

Background

The association between infection with cagA-positive H. pylori and an elevated susceptibility to gastric cancer has been firmly established. PIM2 is known to be overexpressed in various types of cancers; however, the specific mechanism by which cagA influences the regulation of PIM2 expression in gastric cancer remains unidentified at present.

Materials and methods

A mutant NCTC11637ΔcagA strain of H. pylori and the eukaryotic expression vector pcDNA-cagA were constructed for evaluating PIM2 expression levels in gastric cancer cells (HGC27, SGC7901, and AG) co-cultured with the NCTC11637 and NCTC11637ΔcagA strain, as well as pcDNA-cagA and the empty vector pcDNA3.1 (+).

Results

Co-culturing gastric cancer cells with NCTC11637 significantly increased PIM2 ex

SUBMITTER: Liang W 

PROVIDER: S-EPMC11491992 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature