Two-dose priming immunization amplifies humoral immunity by synchronizing vaccine delivery with the germinal center response.

ABSTRACT: Prolonging exposure to subunit vaccines during the primary immune response enhances humoral immunity. Escalating-dose immunization (EDI), administering vaccines every other day in an increasing pattern over 2 weeks, is particularly effective but challenging to implement clinically. Here, using an HIV Env trimer/saponin adjuvant vaccine, we explored simplified EDI regimens and found that a two-shot regimen administering 20% of the vaccine followed by the remaining 80% of the dose 7 days later increased T_FH responses 6-fold, antigen-specific germinal center (GC) B cells 10-fold, and serum antibody titers 10-fold compared with bolus immunization. Computational modeling of T_FH priming and the GC response suggested that enhanced activation/antigen loading on dendritic cell

SUBMITTER: Bhagchandani SH 

PROVIDER: S-EPMC11492009 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature