ZASP: A Highly Compatible and Sensitive ZnCl₂ Precipitation-Assisted Sample Preparation Method for Proteomic Analysis.

ABSTRACT: Universal sample preparation for proteomic analysis that enables unbiased protein manipulation, flexible reagent use, and low protein loss is required to ensure the highest sensitivity of downstream liquid chromatography-mass spectrometry (LC-MS) analysis. To address these needs, we developed a ZnCl₂ precipitation-assisted sample preparation method (ZASP) that depletes harsh detergents and impurities in protein solutions prior to trypsin digestion via 10 min of ZnCl₂ and methanol-induced protein precipitation at room temperature (RT). ZASP can remove trypsin digestion and LC-MS incompatible detergents such as SDS, Triton X-100, and urea at high concentrations in solution and unbiasedly recover proteins independent of the amount of protein input. We demonstrated the se

SUBMITTER: Shao X 

PROVIDER: S-EPMC11492125 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature