Monitoring of intracerebellarly-administered natural killer cells with fluorine-19 MRI.

ABSTRACT:

Purpose

Medulloblastoma (MB) is the most common malignant brain tumor in children. Recent studies have shown the ability of natural killer (NK) cells to lyse MB cell lines in vitro, but in vivo successes remain elusive and the efficacy and fate of NK cells in vivo remain unknown.

Methods

To address these questions, we injected MB cells into the cerebellum of immunodeficient mice and examined tumor growth at various days after tumor establishment via bioluminescence imaging. NK cells were labeled with a fluorine-19 (¹⁹F) MRI probe and subsequently injected either intratumorally or contralaterally to the tumor in the cerebellum and effect on tumor growth was monitored.

Results

The ¹⁹F probe efficiently labeled the NK cells and exhibited little cyto

SUBMITTER: Kennis BA 

PROVIDER: S-EPMC11492566 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature