Transcriptional pathways of terminal differentiation in high- and low-density blood granulocytes in sepsis.

ABSTRACT:

Background

Trauma and infection induce emergency granulopoiesis. Counts of immature granulocytes and transcriptional pathways of terminal granulocytic differentiation in blood are elevated in sepsis but correlate with disease severity. This limits their performance as sepsis biomarkers in critically ill patients. We hypothesized that activation of these pathways in sepsis is attributable to immature low-density (LD) rather than mature high-density (HD) granulocytes.

Methods

We included patients with sepsis and systemic inflammatory response syndrome (SIRS) of comparable disease severity, and additionally septic shock, on intensive or intermediate care unit admission. Blood granulocyte isolation by CD15 MicroBeads was followed by density-gradient centrifugation. Flow cytometr

SUBMITTER: Guenther T 

PROVIDER: S-EPMC11492786 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature