Tracking trash to treasure: <i>in situ</i> monitoring of single microbial cell oil biosynthesis from waste cooking oil using Raman spectroscopy and imaging.

ABSTRACT: Waste cooking oil is a major pollutant that contaminates terrestrial and aquatic bodies which is generated from household kitchens and eateries. The bioremediation of waste cooking oil (WCO) into microbial oil, also known as single microbial cell oil (SMCO), can be accomplished by oleaginous microbes. Conventional methods excel in SMCO analysis but lack efficacy for in situ or lysis-free monitoring of nascent SMCO synthesis and turnover. To bridge this knowledge gap, this study shows the applicability of Raman reverse stable isotope probing (RrSIP) in monitoring time-dependent nascent SMCO synthesis and assimilation in Yarrowia lipolytica, an oleaginous yeast grown in hydrophilic (glucose) as well as hydrophobic carbon sources (cooking oil and waste cooking oil). This study a

SUBMITTER: Karlo J 

PROVIDER: S-EPMC11493132 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature