Pathological autoantibody internalisation in myositis.

ABSTRACT:

Objectives

Autoantibodies targeting intracellular proteins are common in various autoimmune diseases. In the context of myositis, the pathologic significance of these autoantibodies has been questioned due to the assumption that autoantibodies cannot enter living muscle cells. This study aims to investigate the validity of this assumption.

Methods

Confocal immunofluorescence microscopy was employed to localise antibodies and other proteins of interest in myositis muscle biopsies. Bulk RNA sequencing was used to examine the transcriptomic profiles of 669 samples, including those from patients with myositis, disease controls and healthy controls. Additionally, antibodies from myositis patients were introduced into cultured myoblasts through electroporation, and their transcrip

SUBMITTER: Pinal-Fernandez I 

PROVIDER: S-EPMC11493519 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature