FOXO1-mediated autophagy regulation by miR-223 in sepsis-induced immunosuppression.

ABSTRACT:

Introduction

Immunosuppression is the main cause of the high mortality rate in patients with sepsis. The decrease in the number and dysfunction of CD4⁺ T lymphocytes is crucial to the immunosuppressed state of sepsis, in turn affecting the development and prognosis of sepsis. Autophagy has been shown to play an important role in the immune imbalance exhibited during sepsis.

Methods

In this study, we modulate the expression of miR-223 in CD4⁺ T lymphocytes, via the transfection of a mimic or an inhibitor of miR-223 to establish cell models of miR-223 overexpression and knockdown, respectively. Levels of autophagy were monitored using a double-labeled lentivirus (mRFP-GFP-LC3) and electron microscopy, and western blot analysis was used to estimate the lev

SUBMITTER: Xiang G 

PROVIDER: S-EPMC11493625 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature