Navigating directed evolution efficiently: optimizing selection conditions and selection output analysis.

ABSTRACT: Directed evolution is a powerful tool that can bypass gaps in our understanding of the sequence-function relationship of proteins and still isolate variants with desired activities, properties, and substrate specificities. The rise of directed evolution platforms for polymerase engineering has accelerated the isolation of xenobiotic nucleic acid (XNA) synthetases and reverse transcriptases capable of processing a wide array of unnatural XNAs which have numerous therapeutic and biotechnological applications. Still, the current generation of XNA polymerases functions with significantly lower efficiency than the natural counterparts and retains a significant level of DNA polymerase activity which limits their in vivo applications. Although directed evolution approaches are continuously

SUBMITTER: Handal-Marquez P 

PROVIDER: S-EPMC11493728 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature