Successful formation of sperm cells from transplanted primordial germ cells in sterile interspecific avian recipients.

ABSTRACT: Primordial germ cells (PGCs) are stem cells, from which only gametes develop. In birds, the female sex is heterogametic, thus female gene conservation necessitates preservation of PGCs. PGC transplantation can generate germline chimeras in a host organism and develop into gametes. However, competition between host and transplanted PGCs hinder efficiency of germline chimera generation. We hypothezised that in sterile hybrid recipients with no germ cells of its own, transplanted donor PGCs may exclusively form gametes. Advantages of sterile hybrids as host for PGCs is compliant with many national regulations on genetically modified organisms and technically simpler procedure than the use of busulphan. Therefore, we investigated whether sterile interspecific hybrids may be suitable as recipie

SUBMITTER: Molnar M 

PROVIDER: S-EPMC11494066 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature