Histone modifications of circulating nucleosomes are associated with changes in cell-free DNA fragmentation patterns.

ABSTRACT: The analysis of tissues of origin of cell-free DNA (cfDNA) is of research and diagnostic interest. Many studies focused on bisulfite treatment or immunoprecipitation protocols to assess the tissues of origin of cfDNA. DNA loss often occurs during such processes. Fragmentomics of cfDNA molecules has uncovered a wealth of information related to tissues of origin of cfDNA. There is still much room for the development of tools for assessing contributions from various tissues into plasma using fragmentomic features. Hence, we developed an approach to analyze the relative contributions of DNA from different tissues into plasma, by identifying characteristic fragmentation patterns associated with selected histone modifications. We named this technique as FRAGmentomics-based Histone modification A

SUBMITTER: Bai J 

PROVIDER: S-EPMC11494292 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature