Genomic analysis of Drosophila chromosome underreplication reveals a link between replication control and transcriptional territories.

ABSTRACT: In Drosophila polytene chromosomes, most late-replicating regions remain underreplicated. A loss-of-function mutant of the suppressor of underreplication [Su(UR)] gene suppresses underreplication (UR), whereas extra copies of this gene enhance the level and number of regions showing UR. By combining DNA microarray analysis with manipulation of the number of Su(UR) gene copies, we achieved genomic-scale molecular identification of 1,036 genes that are arranged in clusters located in 52 UR chromosomal regions. These regions overlap extensively (96%) but are not completely identical with late-replicating regions of mitotically dividing Kc cells in culture. Reanalysis of published gene expression profiles revealed that genomic regions defined by replication properties include clusters of coord

SUBMITTER: Belyakin SN 

PROVIDER: S-EPMC1149430 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature