An Automated Cell-Free Workflow for Transcription Factor Engineering.

ABSTRACT: The design and optimization of metabolic pathways, genetic systems, and engineered proteins rely on high-throughput assays to streamline design-build-test-learn cycles. However, assay development is a time-consuming and laborious process. Here, we create a generalizable approach for the tailored optimization of automated cell-free gene expression (CFE)-based workflows, which offers distinct advantages over in vivo assays in reaction flexibility, control, and time to data. Centered around designing highly accurate and precise transfers on the Echo Acoustic Liquid Handler, we introduce pilot assays and validation strategies for each stage of protocol development. We then demonstrate the efficacy of our platform by engineering transcription factor-based biosensors. As a model, we rapidly gene

SUBMITTER: Ekas HM 

PROVIDER: S-EPMC11494693 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature