Disruption of mgrB gene by ISkpn14 sourced from a bla_KPC-2 carrying plasmid mediating polymyxin resistance against carbapenem-resistant Klebsiella pneumoniae during treatment: study on the underlying mechanisms.

ABSTRACT:

Background

Carbapenem-resistant Klebsiella pneumoniae (CRKP) infections poses global challenges, with limited options available for targeted therapy. Polymyxin was been regarded as one of the most important last-resort antimicrobial agents. Many factors could accelerate the resistance evolution of polymyxin. Insertion sequence (IS) inserted into mgrB is the main polymyxin resistance mechanism in K. pneumoniae. In this study, two CRKPs (KP31157 and KP31311) were isolated from the urine of a patient, shifting from susceptible to resistant as the mgrB inserted by ISkpn14. We intended to explore the origin of the IS and underlying mechanisms resulting in polymyxin resistance.

Methods

The within-host evolution relationship and molecular features of both CRKPs were determined by p

SUBMITTER: Li Z 

PROVIDER: S-EPMC11494788 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature