Ultrastructural characterization of hippocampal inhibitory synapses under resting and stimulated conditions.

ABSTRACT: The present study uses electron microscopy to document ultrastructural characteristics of hippocampal GABAergic inhibitory synapses under resting and stimulated conditions in three experimental systems. Synaptic profiles were sampled from stratum pyramidale and radiatum of the CA1 region from (1) perfusion fixed mouse brains, (2) immersion fixed rat organotypic slice cultures, and from (3) rat dissociated hippocampal cultures of mixed cell types. Synapses were stimulated in the brain by a 5 min delay in perfusion fixation to trigger an ischemia-like excitatory condition, and by treating the two culture systems with 90 mM high K⁺ for 2-3 min to depolarize the neurons. Upon such stimulation conditions, the presynaptic terminals of the inhibitory synapses exhibited similar structur

SUBMITTER: Tao-Cheng JH 

PROVIDER: S-EPMC11494804 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature