IMPC-based screening revealed that <i>ROBO1</i> can regulate osteoporosis by inhibiting osteogenic differentiation.

ABSTRACT:

Introduction

The utilization of denosumab in treating osteoporosis highlights promising prospects for osteoporosis intervention guided by gene targets. While omics-based research into osteoporosis pathogenesis yields a plethora of potential gene targets for clinical transformation, identifying effective gene targets has posed challenges.

Methods

We first queried the omics data of osteoporosis clinical samples on PubMed, used International Mouse Phenotyping Consortium (IMPC) to screen differentially expressed genes, and conducted preliminary functional verification of candidate genes in human Saos2 cells through osteogenic differentiation and mineralization experiments. We then selected the candidate genes with the most significant effects on osteogenic differentiation and fu

SUBMITTER: Zhang X 

PROVIDER: S-EPMC11494888 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature