Effects of threshold choice on biological conclusions reached during analysis of gene expression by DNA microarrays.

ABSTRACT: Global analysis of gene expression by using DNA microarrays is employed increasingly to search for differences in biological properties between normal and diseased tissue. In such studies, expression that deviates from defined thresholds commonly is used for creating genetic signatures that characterize disease vs. normality. Although it is axiomatic that the threshold parameters applied to microarray analysis will alter the contents of such genetic signatures, the extent to which threshold choice can affect the fundamental conclusions made from microarray-based studies has not been elucidated. We used GABRIEL (Genetic Analysis By Rules Incorporating Expert Logic), a platform of knowledge-based algorithms for the global analysis of gene expression, together with conventional statistical ap

SUBMITTER: Pan KH 

PROVIDER: S-EPMC1149502 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature