Envelope domain III E₃₂₄, E₃₅₁, and E₃₈₀ mutations lever adaptive evolution of DENV-1 genotype I.

ABSTRACT: Dengue virus (DENV) gains genetic mutations during continuous transmission and evolution, making the virus more adaptive and virulent. The clade of DENV-1 genotype I has expanded and become the predominant genotype in Asia and the Pacific areas, but the underlying mechanisms are unclear. A combined analysis of nonsynonymous mutations in domain III of the envelope protein and their biological effects on virus pathogenesis and transmission was evaluated. Phylogenetic analyses found three nonsynonymous mutations (V324I, V351L, and V380I) in domain III of the envelope protein, which emerged in 1970s-1990s and stably inherited and expanded in contemporary strains after 2000. We generated reverse-mutated viruses (I324V, L351V, and I380V) based on an infectious clone of an epidemic DENV-1 strain

SUBMITTER: Jiang T 

PROVIDER: S-EPMC11495034 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature