Azacytidine treatment affects the methylation pattern of genomic and cell-free DNA in uveal melanoma cell lines.

ABSTRACT:

Background

Uveal melanoma (UM) is the most common primary intraocular tumour in adults, and approximately 50% of patients will develop metastasis. Epigenetic changes are a major factor in cancer progression. We aimed to determine whether methylation profiles could be altered using a DNA methyltransferase (DNMT) inhibitor in UM cell lines.

Methods

Four primary and metastatic UM cell lines were treated with azacytidine and analysed for cell proliferation, colony formation, and BAP1 protein expression. Genomic and cell-free (cf)DNA methylation were compared.

Results

In all cell lines, azacytidine treatment resulted in dose-dependent effects on proliferation, colony formation, and radiosensitivity. Methylation profiling revealed differences in methylation between cell lin

SUBMITTER: Ferrier ST 

PROVIDER: S-EPMC11495039 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature