Variable fitness effects of bacteriophage resistance mutations in Escherichia coli: implications for phage therapy.

ABSTRACT: Bacteria exposed to bactericidal treatment, such as antibiotics or bacteriophages (phages), often develop resistance. While phage therapy is proposed as a solution to the antibiotic resistance crisis, the bacterial resistance emerging during phage therapy remains poorly characterized. In this study, we examined a large population of phage-resistant extra-intestinal pathogenic Escherichia coli 536 clones that emerged from both in vitro (non-limited liquid medium) and in vivo (murine pneumonia) conditions. Genome sequencing uncovered a convergent mutational pattern in phage resistance mechanisms under both conditions, particularly targeting two cell-wall components, the K15 capsule and the lipopolysaccharide (LPS). This suggests that their identification in vivo c

SUBMITTER: Gaborieau B 

PROVIDER: S-EPMC11495123 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature