Spatial transcriptomic analysis of adult hippocampal neurogenesis in the human brain.

ABSTRACT:

Background

Adult hippocampal neurogenesis has been extensively characterized in rodent models, but its existence in humans remains controversial. We sought to assess the phenomenon in postmortem human hippocampal samples by combining spatial transcriptomics and multiplexed fluorescent in situ hybridization.

Methods

We computationally examined the spatial expression of various canonical neurogenesis markers in postmortem dentate gyrus (DG) sections from young and middle-aged sudden-death males. We conducted in situ assessment of markers expressed in neural stem cells, proliferative cells, and immature granule neurons in postmortem DG sections from infant, adolescent, and middle-aged males.

Results

We examined frozen DG tissue from infant (n = 1, age 2 yr), adole

SUBMITTER: Simard S 

PROVIDER: S-EPMC11495544 | biostudies-literature | 2024 Sep-Oct

REPOSITORIES: biostudies-literature