Global ¹³C tracing and metabolic flux analysis of intact human liver tissue ex vivo.

ABSTRACT: Liver metabolism is central to human physiology and influences the pathogenesis of common metabolic diseases. Yet, our understanding of human liver metabolism remains incomplete, with much of current knowledge based on animal or cell culture models that do not fully recapitulate human physiology. Here, we perform in-depth measurement of metabolism in intact human liver tissue ex vivo using global ¹³C tracing, non-targeted mass spectrometry and model-based metabolic flux analysis. Isotope tracing allowed qualitative assessment of a wide range of metabolic pathways within a single experiment, confirming well-known features of liver metabolism but also revealing unexpected metabolic activities such as de novo creatine synthesis and branched-chain amino acid transamination, where hu

SUBMITTER: Grankvist N 

PROVIDER: S-EPMC11496108 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature