Study on biotransformation and absorption of genistin based on fecal microbiota and Caco-2 cell.

ABSTRACT:

Introduction

Genistin, as a kind of natural isoflavone glycoside, has good biological activity, and its weak absorption makes it closely related to intestinal flora. However, the role of the intestinal flora is still unclear and whether the metabolites produced by the intestinal flora are absorbed systemically is also variable.

Methods

Genistin was fermented for 24 h based on fecal bacteria fermentation technology. The components were qualitatively and quantitatively analyzed by HPLC and UHPLC-Q-Exactive Orbitrap Mass spectrometry. The composition of intestinal flora in fermentation samples from fecal bacteria was detected by 16S rRNA sequencing. Five representative probiotics were cultured in vitro and fermented with genistin to determine similarities and differences

SUBMITTER: Li Z 

PROVIDER: S-EPMC11496136 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature