Heat stress causes chromatin accessibility and related gene expression changes in crown tissues of barley (Hordeum vulgare).

ABSTRACT: Plant responses to stress caused by high temperatures involve changes occurring at the molecular, metabolic, and physiological levels. Understanding the mechanisms by which plants recognize signals to activate this response is a prerequisite for identifying key genes and signaling pathways and for obtaining heat-tolerant plants. We demonstrated the first implementation of an assay for transposase-accessible chromatin to identify open chromatin regions (OCRs) in crown tissues of barley using three genotypes carrying different allelic forms of the sdw1 gene encoding gibberellin 20-oxidase subjected to elevated temperatures. In parallel, we performed gene expression analysis, which allowed us to relate changes in chromatin state to changes in transcriptional activity. The obtained data reveal

SUBMITTER: Kielbowicz-Matuk A 

PROVIDER: S-EPMC11496342 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature