Transcription-dependent mobility of single genes and genome-wide motions in live human cells.

ABSTRACT: The human genome is highly dynamic across all scales. At the gene level, chromatin is persistently remodeled and rearranged during active processes such as transcription, replication and DNA repair. At the genome level, chromatin moves in micron-scale domains that break up and re-form over seconds, but the origin of these coherent motions is unknown. Here, we investigate the connection between genomic motions and gene-level activity. Simultaneous mapping of single-gene and genome-wide motions shows that the coupling of gene transcriptional activity to flows of the nearby genome is modulated by chromatin compaction. A motion correlation analysis suggests that a single active gene drives larger-scale motions in low-compaction regions, but high-compaction chromatin drives gene motion regardle

SUBMITTER: Chu FY 

PROVIDER: S-EPMC11496510 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature